2QP4

Identification and Characterization of Two Amino Acids Critical for the Substrate Inhibition of SULT2A1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-11
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	109.412, 133.020, 76.303
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
Rwork	0.246
R-free	0.30300
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.900
Number of reflections	12532
Completeness [%]	98.9
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	(NH4)2SO4, NaCl, pH 7.5, vapor diffusion, temperature 291K