2QOO
Human EphA3 kinase and juxtamembrane region, Y596F:Y602F:Y742F triple mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-22 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.111, 38.255, 75.975 |
Unit cell angles | 90.00, 102.24, 90.00 |
Refinement procedure
Resolution | 26.440 - 1.250 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gsf |
RMSD bond length | 0.007 |
RMSD bond angle | 1.107 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.290 |
High resolution limit [Å] | 1.250 | 2.690 | 1.250 |
Rmerge | 0.035 | 0.021 | 0.630 |
Number of reflections | 83007 | ||
<I/σ(I)> | 13.2 | ||
Completeness [%] | 98.4 | 97.5 | 88.7 |
Redundancy | 4 | 4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20 mg/mL Protein, 25% PEG 3350, 0.2M Ammonium sulfate, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |