2QOK

Human EphA3 kinase and juxtamembrane region, Y596F:Y602F:S768A triple mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-22
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	54.046, 38.234, 76.099
Unit cell angles	90.00, 102.31, 90.00

Refinement procedure

Resolution	34.000 - 1.200
R-factor	0.192
Rwork	0.190
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gsf
RMSD bond length	0.006
RMSD bond angle	1.090
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.240
High resolution limit [Å]	1.200	2.590	1.200
Rmerge	0.058	0.045	0.598
Number of reflections	92132
<I/σ(I)>	12.4
Completeness [%]	96.5	97.9	93.6
Redundancy	4.2	4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20 mg/mL Protein, 25% PEG 3350, 0.2M Ammonium sulfate, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K