2QN1
Glycogen Phosphorylase b in complex with asiatic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2007-02-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8088 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.720, 128.720, 116.638 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.910 - 2.401 |
R-factor | 0.18067 |
Rwork | 0.179 |
R-free | 0.20752 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2prj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.129 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.910 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 38832 | |
<I/σ(I)> | 15.5 | 4.2 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 5.3 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10 mM Bes buffer, 3 mM DDT, pH 6.7, SMALL TUBES, temperature 289K |