2QMU
Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.007 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 118.323, 118.323, 161.449 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| R-factor | 0.254 |
| Rwork | 0.254 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | chain A from 2AHO |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.709 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 21823 | |
| <I/σ(I)> | 8.9 | 3.6 |
| Completeness [%] | 98.7 | 99.9 |
| Redundancy | 4.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 1.6M ammonium citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
