2QMH
structure of V267F mutant HprK/P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.059, 106.368, 106.486 |
| Unit cell angles | 119.50, 90.02, 89.96 |
Refinement procedure
| Resolution | 19.430 - 2.600 |
| R-factor | 0.22467 |
| Rwork | 0.221 |
| R-free | 0.29773 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jb1 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.964 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.139 | 0.420 |
| Number of reflections | 81301 | |
| <I/σ(I)> | 13 | 2 |
| Completeness [%] | 99.3 | 97.2 |
| Redundancy | 5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | MPD, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
