2QME
Crystal structure of human RAC3 in complex with CRIB domain of human p21-activated kinase 1 (PAK1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-01-31 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54180 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.226, 53.480, 47.682 |
Unit cell angles | 90.00, 93.45, 90.00 |
Refinement procedure
Resolution | 47.620 - 1.750 |
R-factor | 0.15114 |
Rwork | 0.149 |
R-free | 0.19177 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g0n |
RMSD bond length | 0.013 |
RMSD bond angle | 1.470 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.620 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.053 | 0.343 |
Number of reflections | 21448 | |
<I/σ(I)> | 27.1 | 4 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 7.2 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCl pH 8.5, 2 M (NH4)2S04, VAPOR DIFFUSION, SITTING DROP, temperature 293K |