2QKX
N-acetyl glucosamine 1-phosphate uridyltransferase from Mycobacterium tuberculosis complex with N-acetyl glucosamine 1-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 94.303, 94.303, 288.041 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.420 - 2.750 |
| R-factor | 0.19791 |
| Rwork | 0.195 |
| R-free | 0.25071 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hm9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.673 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.330 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.151 | 0.623 |
| Number of reflections | 13239 | |
| <I/σ(I)> | 13.2 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2M Lithium nitrate, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
