2QKM
The crystal structure of fission yeast mRNA decapping enzyme Dcp1-Dcp2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.289, 161.443, 91.386 |
| Unit cell angles | 90.00, 97.49, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.23611 |
| Rwork | 0.233 |
| R-free | 0.28923 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2A6T 2qkl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.241 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.540 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.130 | 0.323 |
| Number of reflections | 48226 | |
| <I/σ(I)> | 3.3 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 285 | 8% Ethylene Glycol, 100 mM HEPES pH 7.0, 11.6% MPD, 4.4% PEG 4000 and 10 mM ATP, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
