2QIC
Crystal Structure of the ING1 PHD Finger in complex with a Histone H3K4ME3 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2007-02-26 |
Wavelength(s) | 1.257 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 49.060, 49.060, 52.620 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.100 |
Rwork | 0.240 |
R-free | 0.26400 |
Structure solution method | Rigid Body Refinement |
Starting model (for MR) | 2g6q |
RMSD bond length | 0.006 |
RMSD bond angle | 1.091 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.071 | 0.253 |
Number of reflections | 4526 | |
<I/σ(I)> | 16.3 | 5.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.35 | 9.78 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.01 M NiCl2 6H20, 0.1 M Tris, 20% polyethylene glycol monomethyl ether 2K, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |