2QGH
Crystal structure of diaminopimelate decarboxylase from Helicobacter pylori complexed with L-lysine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.248, 79.248, 134.275 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| Rwork | 0.206 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1twi |
| Data reduction software | d*TREK |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.810 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.128 | 0.390 |
| Total number of observations | 15686 | |
| Number of reflections | 21635 | |
| <I/σ(I)> | 5.1 | 1.9 |
| Completeness [%] | 97.0 | 100 |
| Redundancy | 7.14 | 7.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 0.1M Bicine, 1.6M Ammonium Sulfate, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
