2QGA
Plasmodium vivax adenylosuccinate lyase Pv003765 with AMP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.247, 179.545, 68.575 |
| Unit cell angles | 90.00, 99.37, 90.00 |
Refinement procedure
| Resolution | 33.830 - 2.010 |
| R-factor | 0.17693 |
| Rwork | 0.175 |
| R-free | 0.21013 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hvg |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.531 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Number of reflections | 93338 | |
| <I/σ(I)> | 13.48 | 2.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 2M Ammonia Sulfate, 0.2M K/Na Tartrate, 0.1M Na Citrate pH5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
