2QFI
Structure of the zinc transporter YiiP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 106.660, 110.839, 130.662 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 3.800 |
R-factor | 0.32259 |
Rwork | 0.322 |
R-free | 0.32934 |
Structure solution method | MIR |
RMSD bond length | 0.027 |
RMSD bond angle | 2.834 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 4.000 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.129 | 0.429 |
Number of reflections | 15534 | |
<I/σ(I)> | 13.3 | 1.9 |
Completeness [%] | 97.3 | 91.9 |
Redundancy | 6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |