2QFI
Structure of the zinc transporter YiiP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 106.660, 110.839, 130.662 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.800 |
| R-factor | 0.32259 |
| Rwork | 0.322 |
| R-free | 0.32934 |
| Structure solution method | MIR |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.834 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.000 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.129 | 0.429 |
| Number of reflections | 15534 | |
| <I/σ(I)> | 13.3 | 1.9 |
| Completeness [%] | 97.3 | 91.9 |
| Redundancy | 6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
