2QFB
Crystal structure of the regulatory domain of human RIG-I with bound Zn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.87300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.900, 76.600, 139.400 |
| Unit cell angles | 90.00, 93.30, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.257 |
| Rwork | 0.248 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qfd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.520 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 40897 | |
| <I/σ(I)> | 8.97 | 2.13 |
| Completeness [%] | 98.2 | 98 |
| Redundancy | 2.54 | 2.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10 | 293.15 | 0.1 M CHES, 18% w/v PEG 8000, pH 10.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
