2QEQ
Crystal structure of kunjin virus ns3 helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.129, 38.095, 148.113 |
| Unit cell angles | 90.00, 96.98, 90.00 |
Refinement procedure
| Resolution | 39.750 - 3.100 |
| R-factor | 0.24738 |
| Rwork | 0.243 |
| R-free | 0.33058 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bmf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.991 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.153 | 0.609 |
| Number of reflections | 17953 | |
| <I/σ(I)> | 10.9 | 3.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 4000 10%, Na Acetate 0.2 M, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
