2QEK
HIV-1 subtype F DIS RNA extended duplex form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2007-04-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.773, 37.355, 44.965 |
Unit cell angles | 90.00, 111.24, 90.00 |
Refinement procedure
Resolution | 21.690 - 1.800 |
R-factor | 0.2511 |
Rwork | 0.251 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y99 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 24275 | |
<I/σ(I)> | 19.3 | 6.6 |
Completeness [%] | 1.0 | 0.998 |
Redundancy | 6 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 310 | MPD, MgCl2, KCl, Na cacodylate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Na cacodylate | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | KCl | ||
5 | 1 | 2 | MPD | ||
6 | 1 | 2 | MgCl2 | ||
7 | 1 | 2 | KCl |