2QE3
Crystal structure of human tl1a extracellular domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-06 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 120.404, 120.404, 120.404 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.800 - 2.500 |
| R-factor | 0.21085 |
| Rwork | 0.209 |
| R-free | 0.26336 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tnf |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.355 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.170 | 0.560 |
| Number of reflections | 10856 | |
| <I/σ(I)> | 3.5 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 21.1 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | 10 MM CACL2, 100 MM TRIS, PH 8.50, 20% METHONAL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K, temperature 298.0K |
