2QCD
Crystal structure of the orotidine-5'-monophosphate decarboxylase domain of human UMP synthase bound to UMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.394, 61.643, 69.970 |
| Unit cell angles | 90.00, 112.71, 90.00 |
Refinement procedure
| Resolution | 42.260 - 2.030 |
| R-factor | 0.15174 |
| Rwork | 0.149 |
| R-free | 0.19460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.388 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | COMO |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.300 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 34716 | |
| <I/σ(I)> | 18.6 | 3.9 |
| Completeness [%] | 98.0 | 83.8 |
| Redundancy | 6.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
