2QB0

Structure of the 2TEL crystallization module fused to T4 lysozyme with an Ala-Gly-Pro linker.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	P 32
Unit cell lengths	122.620, 122.620, 53.586
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.560
R-factor	0.213
Rwork	0.211
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ji7
RMSD bond length	0.006
RMSD bond angle	1.419
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.000	90.000	2.640
High resolution limit [Å]	2.550	5.490	2.550
Rmerge	0.062	0.028	0.479
Number of reflections	29148
<I/σ(I)>	17.6
Completeness [%]	100.0	100	100
Redundancy	5	5	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1