2Q8K
The crystal structure of Ebp1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.887, 73.887, 183.348 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.540 - 1.600 |
| R-factor | 0.18416 |
| Rwork | 0.182 |
| R-free | 0.21852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1WKM.pdb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.229 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0008) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 67896 | |
| <I/σ(I)> | 12.9 | 2.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 2.3 M Ammoniumsulfate, 0.1M Citric acid, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
