2Q7N
Crystal structure of Leukemia inhibitory factor in complex with LIF receptor (domains 1-5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.968 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 193.476, 240.131, 202.631 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 4.000 |
| R-factor | 0.23978 |
| Rwork | 0.237 |
| R-free | 0.28696 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1PVH CHAIN B |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.739 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.226 | 0.857 |
| Number of reflections | 37104 | |
| <I/σ(I)> | 6.3 | 1 |
| Completeness [%] | 94.2 | 72.5 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1.5M sodium malonate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
