2Q62
Crystal Structure of ArsH from Sinorhizobium meliloti
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-07-26 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.000 |
Spacegroup name | P 42 |
Unit cell lengths | 158.534, 158.534, 87.953 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.630 - 1.800 |
R-factor | 0.175 |
Rwork | 0.173 |
R-free | 0.21200 |
Structure solution method | SIR |
RMSD bond length | 0.016 |
RMSD bond angle | 1.652 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SnB |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.633 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.088 | 0.532 |
Total number of observations | 227608 | |
Number of reflections | 176155 | |
<I/σ(I)> | 5.7 | 1.5 |
Completeness [%] | 92.1 | 83.5 |
Redundancy | 11.4 | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 17% PEG 6000, 0.1 M calcium acetate, 0.1 M cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |