2Q5D
Crystal Structure of Human Importin Beta bound to the Snurportin1 IBB-domain second crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | .97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.985, 100.696, 108.680 |
| Unit cell angles | 90.00, 109.23, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.200 |
| Rwork | 0.303 |
| R-free | 0.32800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qgk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 50424 | |
| <I/σ(I)> | 1.72 | |
| Completeness [%] | 78.3 | 51.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 285 | 22%PEG 8000, 0.05M SODIUM CHLORIDE 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
