2Q3H
The crystal structure of RhouA in the GDP-bound state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.03315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.068, 53.600, 54.128 |
| Unit cell angles | 90.00, 118.80, 90.00 |
Refinement procedure
| Resolution | 47.460 - 1.730 |
| R-factor | 0.18474 |
| Rwork | 0.183 |
| R-free | 0.22198 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology based model |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.686 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.460 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.033 | 0.490 |
| Number of reflections | 21530 | |
| Completeness [%] | 99.6 | 98 |
| Redundancy | 4.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2M MgCl2; 0.1M TRIS; 25% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
