2Q3B
1.8 A Resolution Crystal Structure of O-Acetylserine Sulfhydrylase (OASS) Holoenzyme From MYCOBACTERIUM TUBERCULOSIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-09-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 70.991, 70.991, 179.624 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.960 - 1.800 |
| R-factor | 0.17508 |
| Rwork | 0.174 |
| R-free | 0.19247 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z7w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.460 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.094 | 0.542 |
| Number of reflections | 43621 | |
| <I/σ(I)> | 16.4 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.1 M HEPES, 80% MPD, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
