2Q3A
Crystal Structure of Rhesus Macaque CD8 Alpha-Alpha Homodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-02-05 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.543, 56.259, 82.314 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.850 - 2.200 |
Rwork | 0.213 |
R-free | 0.25660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1akj |
RMSD bond length | 0.006 |
RMSD bond angle | 1.335 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.136 | 0.637 |
Number of reflections | 11313 | |
<I/σ(I)> | 2.6 | |
Redundancy | 6.6 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291.15 | 0.05 M potassium phosphate monobasic, 20% w/v Polyethylene glycol 8,000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |