2Q2E
Crystal structure of the topoisomerase VI holoenzyme from Methanosarcina mazei
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2006-03-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1159, 1.2386, 1.21495, 1.21434 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 227.812, 227.812, 208.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 4.000 |
| R-factor | 0.3079 |
| Rwork | 0.306 |
| R-free | 0.34927 |
| Structure solution method | MAD |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.3.0026) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 4.220 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Number of reflections | 25975 | |
| <I/σ(I)> | 9.1 | 1.8 |
| Completeness [%] | 95.2 | 96.8 |
| Redundancy | 5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 100 mM sodium cacodylate pH 6.0, 1.6-1.8 M ammonium sulfate pH 7.5, 10 mM magnesium chloride, 10 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
