2Q21

CRYSTAL STRUCTURES AT 2.2 ANGSTROMS RESOLUTION OF THE CATALYTIC DOMAINS OF NORMAL RAS PROTEIN AND AN ONCOGENIC MUTANT COMPLEXED WITH GSP

Replaces:  3P21

Experimental procedure

Spacegroup name	P 65 2 2
Unit cell lengths	83.200, 83.200, 105.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.192 *
Rwork	0.192
RMSD bond length	0.024
RMSD bond angle	2.500
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.081 *
Total number of observations	48073 *
Number of reflections	10447 *
Completeness [%]	80.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *		took Jancarik et al., 1988 from original paper *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Hepes	75 (mM)
2	1	1		100 (mM)
3	1	1	EDTA	0.5 (mM)
4	1	1	dithiothreitol	0.5 (mM)
5	1	1	n-octylglucoside	0.005 (%)
6	1	2	PEG400	30 (%)