2Q1E
Altered dimer interface decreases stability in an amyloidogenic kappa1 Bence Jones protein.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-07 |
Detector | SBC-3 |
Wavelength(s) | 1.0718 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 176.054, 176.054, 176.054 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.210 - 2.550 |
R-factor | 0.1662 |
Rwork | 0.165 |
R-free | 0.20601 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Vk1 O18/O8 germline variable light chain domain monomer |
RMSD bond length | 0.020 |
RMSD bond angle | 1.832 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 176.000 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 29819 | |
<I/σ(I)> | 82.5 | 9.8 |
Completeness [%] | 99.3 | 93 |
Redundancy | 98.7 | 68.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | Mother Liquor: 15-30% (w/v) PEG 4K, 0.2 M Li2SO4, 0.1 M TRIS pH 7.9-8.9. Protein: 890 micromolar in 10 mM TRIS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |