2PYO
Drosophila nucleosome core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.030, 182.040, 109.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 500.000 - 2.430 |
| Rwork | 0.229 |
| R-free | 0.26200 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.195 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.500 | |
| High resolution limit [Å] | 2.430 | 10.000 | 2.430 |
| Rmerge | 0.062 | 0.024 | 0.482 |
| Number of reflections | 75234 | ||
| <I/σ(I)> | 12.26 | 32.9 | 1.6 |
| Completeness [%] | 93.6 | 57.2 | 75.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | Crystallization was carried out by equilibrating a droplet containing 3 mg/ml nucleosome core particle, 80-85 mM MnCl2, 50-80 mM KCl and 20 mM potassium cacodylate (pH 6.0) against a reservoir solution containing of 40-42.5 mM MnCl2, 25-40 mM KCl and 20 mM potassium cacodylate (pH 6.0). , VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 80-85 mM MnCl2 | |||
| 2 | 1 | 50-80 mM KCl | |||
| 3 | 1 | potassium cacodylate | |||
| 4 | 1 | 40-42.5 mM MnCl2 | |||
| 5 | 1 | 25-40 mM KCl | |||
| 6 | 1 | potassium cacodylate |
