2PYI
Crystal structure of Glycogen Phosphorylase in complex with glucosyl triazoleacetamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8073 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 125.934, 125.934, 115.287 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.880 |
| R-factor | 0.20176 |
| Rwork | 0.200 |
| R-free | 0.23839 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | PBD entry 2PYD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.995 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 71717 | |
| <I/σ(I)> | 24.5 | 6.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10 MM BES BUFFER, 0.1 MM EDTA, 0.02% NAN3, 3 MM DTT, pH 6.7, SMALL TUBES, temperature 289K |
