2PYD
The crystal structure of Glycogen phosphorylase in complex with glucose at 100 K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-03 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8068 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 125.730, 125.730, 114.933 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.930 |
R-factor | 0.1926 |
Rwork | 0.190 |
R-free | 0.23500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1ww2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.103 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.960 |
High resolution limit [Å] | 1.930 | 1.930 |
Number of reflections | 65895 | |
<I/σ(I)> | 18.2 | 4.2 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 4.9 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10 MM BES BUFFER, 0.1 MM EDTA,0.02% NAN3, 3 MM DTT, pH 6.7, SMALL TUBES, temperature 289K |