2PXR

Crystal Structure of HIV-1 CA146 in the Presence of CAP-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-14
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	42.187, 62.777, 106.286
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.150 - 1.500
R-factor	0.166
Rwork	0.163
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.873
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.150	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.049	0.318
Total number of observations		5356
Number of reflections	21930
<I/σ(I)>	5.6	2.2
Completeness [%]	95.7	78.5
Redundancy	2.9	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	286	100 mM Tris pH, 5% PEG 8000, 20% PEG 300, 10% glycerol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K