2PXJ
The complex structure of JMJD2A and monomethylated H3K36 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 100.863, 150.172, 57.317 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.560 - 2.000 |
| Rwork | 0.256 |
| R-free | 0.27620 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2gp3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.034 | 0.535 |
| Number of reflections | 58067 | |
| <I/σ(I)> | 18.1 | 1.2 |
| Completeness [%] | 97.3 | 78.5 |
| Redundancy | 8.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 15-17% PEG 5000 mme, pH 8.5, VAPOR DIFFUSION, HANGING DROP |
