2PXI
Ternary complex of DNA polymerase beta with a dideoxy terminated primer and 2'-deoxyguanosine 5'-beta, gamma-monofluoromethylene triphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-05-26 |
Detector | RIGAKU |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.600, 80.200, 55.400 |
Unit cell angles | 90.00, 107.80, 90.00 |
Refinement procedure
Resolution | 24.090 - 2.100 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fmp |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.119 | 0.445 |
Number of reflections | 22466 | |
<I/σ(I)> | 3 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 50 mM Imidazole, 350 mM Sodium Acetate, PEG 3350 16%, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Imidazole | ||
2 | 1 | 1 | Sodium Acetate | ||
3 | 1 | 1 | PEG 3350 | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | Imidazole | ||
6 | 1 | 2 | Sodium Acetate | ||
7 | 1 | 2 | PEG 3350 | ||
8 | 1 | 2 | H2O |