2PWT
Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.490, 43.020, 98.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.000 - 1.800 |
Rwork | 0.211 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j7t |
RMSD bond length | 0.006 |
RMSD bond angle | 0.900 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.400 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.070 | 0.609 |
Number of reflections | 14605 | |
Completeness [%] | 100.0 | |
Redundancy | 19.1 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 310 | 50 mM Na Cacodylate (pH 6.4), 150 mM KCl, 1% Glycerol, 1% 2-methyl-2,4-pentandiol, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | Glycerol | ||
4 | 1 | 1 | sodium Cacodylate | ||
5 | 1 | 2 | MPD | ||
6 | 1 | 2 | KCl | ||
7 | 1 | 2 | Glycerol |