2PVS
Structure of human pancreatic lipase related protein 2 mutant N336Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-09 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 216.181, 216.181, 123.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.180 - 3.000 |
| R-factor | 0.1926 |
| Rwork | 0.190 |
| R-free | 0.24948 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gpl |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.369 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.118 | 0.480 |
| Number of reflections | 27791 | |
| <I/σ(I)> | 10.5 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 6 microliter of protein (14.5 mg/ml in 0.2 M NaCl, 25 mM Tris-HCl, pH 8.0) and 2 microliter of 2.05 M Ammonium sulphate, 0.1 M Hepes, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
