2PSW
Human MAK3 homolog in complex with CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.643, 102.913, 67.157 |
| Unit cell angles | 90.00, 105.81, 90.00 |
Refinement procedure
| Resolution | 40.260 - 2.100 |
| R-factor | 0.2006 |
| Rwork | 0.197 |
| R-free | 0.26463 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ob0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.512 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.260 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 36244 | |
| <I/σ(I)> | 15.9 | 2.3 |
| Completeness [%] | 98.7 | 98.6 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Reservoir: 0.1 M Sodium acetate pH 5.0, 16% PEG3350, 10 mM DTT, 0.1% Dioxane. Protein: 11.8 mg/mL + 3-fold molar excess of CoA in 20 mM HEPES pH 7.5, 0.1 M NaCl, 2.5% 1,2-Propanediol, and 10 mM DTT. Cryo: 75% Reservoir + 25% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
