2PRT
Structure of the Wilms Tumor Suppressor Protein Zinc Finger Domain Bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 97 |
| Collection date | 2002-02-01 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 86.879, 86.879, 171.202 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 3.150 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aay |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 7166 | |
| <I/σ(I)> | 22.9 | 5.3 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 11.2 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 277 | 10 mM d11-Tris/HCl, 20 mM KCl, 5uM ZnSO4, 2mM NaN3, 10% D2O, pH 6.7, SMALL TUBES, temperature 277K, pH 6.70 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | d11-Tris/HCl | ||
| 2 | 1 | 2 | d11-Tris/HCl | ||
| 3 | 1 | 1 | KCl | ||
| 4 | 1 | 2 | KCl | ||
| 5 | 1 | 1 | ZnSO4 | ||
| 6 | 1 | 2 | ZnSO4 | ||
| 7 | 1 | 1 | NaN3 | ||
| 8 | 1 | 2 | NaN3 |
