2PRT
Structure of the Wilms Tumor Suppressor Protein Zinc Finger Domain Bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 97 |
Collection date | 2002-02-01 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 86.879, 86.879, 171.202 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | ? - 3.150 |
R-factor | 0.236 |
Rwork | 0.236 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aay |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.800 | |
High resolution limit [Å] | 3.150 | 3.150 |
Number of reflections | 7166 | |
<I/σ(I)> | 22.9 | 5.3 |
Completeness [%] | 99.1 | 100 |
Redundancy | 11.2 | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.7 | 277 | 10 mM d11-Tris/HCl, 20 mM KCl, 5uM ZnSO4, 2mM NaN3, 10% D2O, pH 6.7, SMALL TUBES, temperature 277K, pH 6.70 |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | d11-Tris/HCl | ||
2 | 1 | 2 | d11-Tris/HCl | ||
3 | 1 | 1 | KCl | ||
4 | 1 | 2 | KCl | ||
5 | 1 | 1 | ZnSO4 | ||
6 | 1 | 2 | ZnSO4 | ||
7 | 1 | 1 | NaN3 | ||
8 | 1 | 2 | NaN3 |