2PQ5
Crystal structure of Dual specificity protein phosphatase 13 (DUSP13)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.03310 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 73.821, 73.821, 303.803 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.1765 |
| Rwork | 0.176 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gwo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.022 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.088 | 0.598 |
| Number of reflections | 43981 | |
| <I/σ(I)> | 15 | 2 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 5.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 25% PEG3350, 0.15 M Ammonium citrate pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
