2PNZ
Crystal structure of the P. abyssi exosome RNase PH ring complexed with UDP and GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.427 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 93.810, 93.810, 126.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.870 - 2.140 |
| R-factor | 0.19088 |
| Rwork | 0.188 |
| R-free | 0.25481 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ba0 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.312 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.980 | 2.260 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 36060 | |
| <I/σ(I)> | 11.3 | 3.3 |
| Completeness [%] | 99.3 | 96 |
| Redundancy | 14.8 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1 M Bis-Tris, 45% MPD and 0.1 M LiCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
