2PMF

The crystal structure of a human glycyl-tRNA synthetase mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Detector technology	CCD
Collection date	2006-07-13
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	91.412, 91.412, 246.814
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.240 - 2.850
R-factor	0.233
Rwork	0.231
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	The native T.thermophilus GlyRS structure
RMSD bond length	0.003
RMSD bond angle	0.847
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.066	0.370
Number of reflections	25080
<I/σ(I)>	15
Completeness [%]	99.1	98.5
Redundancy	13.8	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1