2PMD

The structures of aIF2gamma subunit from the archaeon Sulfolobus solfataricus in the GDP-bound form.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 31
Unit cell lengths	95.060, 95.060, 165.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.850 - 2.650
R-factor	0.22
Rwork	0.220
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	gamma subunit form the aIF2 alpha-gamma dimer. PDB ENTRY: 2AHO
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.850	2.810
High resolution limit [Å]	2.650	2.650
Rmerge	0.076
Number of reflections	45642
<I/σ(I)>		2.58
Completeness [%]	94.3	85.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	295	natrium malonate dihydrate, glycine,CdCl2, GDPNP., pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K