2PLZ
Arg-modified human beta-defensin 1 (HBD1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 20.366, 27.408, 55.079 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.360 |
| R-factor | 0.10776 |
| Rwork | 0.105 |
| R-free | 0.15797 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2IJV |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.963 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.410 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.087 | 0.341 |
| Number of reflections | 7002 | |
| <I/σ(I)> | 17 | 4.2 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 5.7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.0 M Lithium sulfate, 2% (w/v) PEG8000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
