2PKO

Crystal structure of yeast Urm1 at 1.8 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-29
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	P 21 21 2
Unit cell lengths	50.871, 62.681, 27.244
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.750 - 1.800
R-factor	0.19175
Rwork	0.188
R-free	0.26295
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xo3
RMSD bond length	0.012
RMSD bond angle	1.419
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.750
High resolution limit [Å]	1.800	1.800
Number of reflections	7848
Completeness [%]	97.2	97.16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	33-35% PEG 8000, 0.1M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K