2PK9
Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2005-07-21 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97948 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 147.775, 147.775, 211.825 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.030 - 2.906 |
R-factor | 0.28316 |
Rwork | 0.281 |
R-free | 0.31536 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.469 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.030 | 3.000 |
High resolution limit [Å] | 2.906 | 2.906 |
Rmerge | 0.096 | 0.290 |
Number of reflections | 47431 | |
<I/σ(I)> | 12.2 | 2.4 |
Completeness [%] | 88.4 | 71.2 |
Redundancy | 3.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | 9ul 12% PEG 10000, 10% glycerol, 0.2ul 0.25 M strontium chloride (SrCl2), 10 mM Tris (2-carboxyethyl) phosphine (TCEP, 0.1 M 2-morpholinoethanesulfonic acid (MES), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | 9ul 12% PEG 10000, 10% glycerol, 0.2ul 0.25 M strontium chloride (SrCl2), 10 mM Tris (2-carboxyethyl) phosphine (TCEP, 0.1 M 2-morpholinoethanesulfonic acid (MES), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |