2PHN
Crystal structure of an amide bond forming F420-gamma glutamyl ligase from Archaeoglobus fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97940 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 98.367, 98.367, 94.347 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.350 |
R-factor | 0.16179 |
Rwork | 0.160 |
R-free | 0.18997 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g9i |
RMSD bond length | 0.012 |
RMSD bond angle | 1.564 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.055 | 0.490 |
Number of reflections | 99727 | |
<I/σ(I)> | 22.3 | 4.69 |
Completeness [%] | 98.7 | 97.9 |
Redundancy | 8.2 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 0.2 M Ammonium acetate, 0.1 M Sodium citrate, 25% PEG 5000 MME, 10 mM GDP, MnCl2, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |