2PG8
Crystal structure of R254K mutanat of DpgC with bound substrate analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 139.054, 155.309, 169.208 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| Rwork | 0.331 |
| R-free | 0.36700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2np9 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.158 | 0.749 |
| Number of reflections | 73630 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 9.8 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 15% PEG 4000, 0.1M sodium citrate, 0.15M ammonium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
