2PG1

Structural analysis of a cytoplasmic dynein Light Chain-Intermediate Chain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	116.969, 119.870, 211.702
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.800
Rwork	0.208
R-free	0.25300
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.03)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.085
Number of reflections	36881
<I/σ(I)>	31	6.7
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	1.8 M - 2.2 M Ammonium Sulfate, 0 - 20 % glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K