2PF4
Crystal structure of the full-length simian virus 40 small t antigen complexed with the protein phosphatase 2A Aalpha subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-17 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 |
Unit cell lengths | 94.711, 105.501, 111.937 |
Unit cell angles | 115.64, 109.55, 94.11 |
Refinement procedure
Resolution | 20.000 - 3.100 |
R-factor | 0.247 |
Rwork | 0.244 |
R-free | 0.30400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2IAE-A |
RMSD bond length | 0.009 |
RMSD bond angle | 1.387 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.076 | 0.758 |
Number of reflections | 62693 | |
<I/σ(I)> | 12.1 | 1.8 |
Completeness [%] | 98.0 | 88.7 |
Redundancy | 7.1 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 298 | 16% PEG 3350, 0.2 M Ammonium formate, 30 mM spermine, 6% 6-aminocaproic acid, 10 mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 8.00 |